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Hanna HI-5421 Research Grade DO & BOD Bench Meter


HI-5421 is a research-grade benchtop meter with extensive capabilities in measuring DO as well as BOD, OUR, SOUR and atmospheric pressure and temperature.

Dissolved Oxygen
2 years manufacturers' warranty

£1,097.50 (exc.VAT)
£1,317.00 (tax incl.)
Order now for 2 weeks delivery
Full Description Specification Manuals

Ideal for:

  • University research
  • Pharmaceutical research & development
  • Food and drink including wine analysis
  • Water pollution control

HI-5421 is a research-grade benchtop meter with extensive capabilities in measuring DO as well as BOD, OUR, SOUR and atmospheric pressure and temperature. It has a large, graphic LCD, backlit for improved clarity and a user-friendly soft touch keypad.

This is a highly advanced instrument featuring an intuitive menu system to streamline the workflow process and provide accurate measurements quickly and efficiently.

DO measurements can be performed in ppm (mg/L) range or in % and feature automatic or manual temperature and atmospheric pressure compensation and manual salinity compensation.

Automatic and manual calibration of dissolved oxygen can be performed in one or two points using standard or user-defined standards.

Users can set the reading stability criteria choosing from three modes: Fast; Medium; or Accurate. When measurement stability is reached, the direct/autohold feature automatically holds the reading on the display. In direct reading mode, the user interface can be set to basic with or without GLP, graphic or logging information.

Key features

  • Extended DO range up to 90ppm (mg/L) and 600%
  • Three salinity scales: practical salinity, natural sea water, percent
  • Measures DO, atmospheric pressure & temperature
  • DO direct, DO direct/autohold, BOD: Biological Oxygen Demand, OUR: Oxygen Uptake Rate, SOUR: Specific Oxygen Uptake Rate
  • Temperature and atmospheric pressure automatic compensation
  • Manual salinity compensation
  • Large log memory with different logging methods
  • Polarographic probe with built-in temperature sensor
  • Improved backlit display
  • Capacitive touch key pad
  • Clean user interface
  • On-screen help

Advanced measurement

The HI-5421 can be used to perform a full range of DO, BOD, OUR and SOUR measurements together with atmospheric pressure and temperature.

The DO probe uses the polarographic principal of measurement and has a built-in temperature sensor. The BOD, OUR and SOUR methods are supported with a dedicated tutorial and step by step guide to ensure a full and correct reading of DO using this specific measuring method.

Up to 10 profiles can be saved and recalled eliminating the need to reconfigure each time a different electrode is used. User definable configurations can include:

Reading mode

  • direct or BOD, OUR and SOUR measurement units
  • temperature units
  • stability criteria


  • temperature
  • atmospheric pressure
  • salinity

Three selectable logging modes are available: Automatic, Manual and AutoHold logging. Up to 100 logging lots can be stored for automatic or manual modes. Automatic logging also features a selectable area and sampling period.

GLP informatics includes complete data about user calibration of each parameter and identification information for the instrument, user and company.

Data can be transferred to a PC via the opto-isolated PC interface via the RS232 or USB ports and HI-92000 Windows compatible software.

All features are available in English, Italian, Portuguese and Spanish. Contextual help is available through a dedicated HELP key with clear tutorial messages and directions available on-screen. This guides users quickly and clearly through the procedures to facilitate accurate measurement and calibration.

Supplied with

  • HI-76483 DO probe
  • HI-76404N electrode holder
  • HI-7041S electrolyte solution (30mL)
  • DO membrane caps (2)
  • 12 VDC adapter
  • Quality certificate
  • Instruction manual

Read More

DO - Range0.00 to 90.00 ppm; 0.0 to 600.0% saturation
 Resolution0.01 ppm; 0.1% saturation
 Accuracy±1.5% of reading ±1 digit
 Calibrationautomatic using single or two-point calibration; user calibration single point
Barometric Pressure - Range450 to 850 mmHg; 560 to 1133 mBar (additional units available (kPa, mHg, psi, atm))
 Resolution1 mm Hg
 Accuracy±3 mmHg within + 1 least significant digit
 Calibrationsingle point calibration
Temperature - Range-20.0 to 120°C
  Accuracy±0.2°C (without probe)
Measurement ModesDirect D.O.; BOD (Biochemical Oxygen Demand); OUR (Oxygen Uptake Rate); SOUR (Specific Oxygen Uptake Rate)
Temperature Compensation0.0 to 50.0°C
Salinity Compensation0 to 45 g/L (ppt), % or PSU
ProbeHI-76483 thin body, polarographic dissolved oxygen probe with internal temperature sensor and 1m cable (included)
Logging - Record samples100,000 records storage, 100 lots each for automatic and manual logs; maximum 50,000 records/log for automatic logging
 Logging intervalfourteen intervals available from 1 second to 180 minutes/interval
 TypeManual AutoHOLD, automatic
Alarm (DO, BOD, OUR, SOUR)inside and outside limits
PC Connectionopto-isolated USB
Displaygraphic colour LCD with on-screen help, graphing, language selection and custom configuration
Power Supply12 VDC adapter (included)
Dimensions/Weight160 x 231 x 94 mm/ 1.2 kg